A Segment Of The Will Die Slowly Gene From Drosophila...

Title: â€Å"PCR amplification, TA cloning, sequencing and sequence analysis of a segment of the will die slowly gene from Drosophila melanogaster.† Abstact: A seemingly novel gene sequence has been discovered in fruit flies from Nicholas Harden’s lab at Simon Fraser University using random PCR primers. Attempts at purifying and characterizing this gene have been elusive. Here, we show methods for isolating, amplifying, and purifying the gene of interest for analysis. Using polymerase chain reaction to amplify the gene, it is then ligated into a pGEM-T Easy vector for TA cloning experiments. Transformation for further vector proliferation is done on competent Escherichia coli cells. Subsequently, the vector is purified through plasmid preparation and sent to an off-site location for sequencing. Upon sequence analysis, it is suggested that the gene of interest, will die slowly, belongs to the WD repeat WDR5/wds family, and has implications in histone acetyltransferase activity. Introduction: Research is a slow and meticulous process required to understand the smallest details of the universe. That is why, in order to study an organism, we must delve deep into their molecular biology. With that said, to understand the processes of the common fruit fly, Drosophila melanogaster, we must look at their genome to which their function comes from. So with the discovery a new gene, we must be able to isolate, amplify, purify, and characterize it. To do this, many techniques of